CALLOGENESIS AND PLANT REGENERATION VIA IN VITRO CULTURE OF STEVIA REBAUDIANA EXPLANTS

doi:10.12816/ejpb.2019.256166

CALLOGENESIS AND PLANT REGENERATION VIA IN VITRO CULTURE OF STEVIA REBAUDIANA EXPLANTS

Document Type : Original research

10.12816/ejpb.2019.256166

Abstract

Tissue culture technique became one of the most important tools in plant breeding. Callus is important for rapid mass multiplication, generation of variability, cell suspension culture, preservation of cell line culture and production of secondary metabolites. Therefore, this study was carried out on stevia plant for establishing efficient methods of formation and regeneration of callus. Three different explants (shoot tip, leaves and nodal segments) and Murashige and Skoog medium (MS) with different concentrations and combinations of growth regulators (PGRs) were used to determine which explant is the most suitable for callus induction and regeneration in the presence of different PGRs. All studied media induced callus for all explants, but MSc₄ (MS+1.0 mgl^-1 2,4-D +0.75 mgl^-1 NAA) gave the highest values of callus fresh weight. Only, calli obtained from Msc₄ callus induction medium gave the best response to regenerate a sufficient number of shoots. Half strength MS medium with 1 mgl^-1IBA was found to be the optimum medium for root formation. It gave a good root formation (88.67%), highest roots number/shoot (6.24), and highest root length (2.90cm).

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