SELECTING DIVERSE BREAD WHEAT GENOTYPES UNDER SALINE STRESS CONDITIONS USING ISSR MARKER AND TOLERANCE INDICES

doi:10.12816/ejpb.2019.256167

SELECTING DIVERSE BREAD WHEAT GENOTYPES UNDER SALINE STRESS CONDITIONS USING ISSR MARKER AND TOLERANCE INDICES

Document Type : Original research

10.12816/ejpb.2019.256167

Abstract

The study aimed at identifying and assessing salt tolerance and genetic diversity of five wheat genotypes in two diverse locations (Moshtohor and Siwa) during the winter season of 2016/2017. A total of 46 amplified fragments were generated by the five primers, 54.35% of them were polymorphic. High polymorphism (63.64%) was observed by using primer 17899A. 7 and 11 unique bands were detected in positive and negative states, respectively. These bands distinguished the genotypes for their tolerance to stress. Highest genetic diversity of 40.5% was obtained between Shandweel-1 and Misr-1 genotypes, whereas, the lowest one of 20.4% was between Nesr and S8/17 genotypes. Total number of effective alleles was 82.34. The polymorphic information content per primer ranged from 0.885 for 17898B primer to 0.959 for 17899B primer with an average of 0.922. Grains yield m^-2 was significantly different between the stress conditions (Siwa) and normal conditions (Moshtohor). Differences varied among wheat genotypes. The highest grain yield by S8/17 (S8 tissue culture regenerated doubled haploid line) followed by Misr-1. Stress tolerance index (STI) and grain yield were the highest for line S8/17 and the lowest for Misr-2. Similar ranks for such genotypes were observed by geometric mean productivity (GMP) and Harmonic mean (HM) parameters as well as for yield index (YI). Nesr genotype which showed the highest stress tolerance (TOL) and stress susceptibility index (SSI) as well as yield stability index (YSI) suggests more stress tolerance mechanisms in this genotype.

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