GENETIC RELATIONSHIPS AMONG TEN SUNFLOWER INBRED LINES BASED ON ISSR AND RAPD ANALYSES

doi:10.12816/ejpb.2019.344255

GENETIC RELATIONSHIPS AMONG TEN SUNFLOWER INBRED LINES BASED ON ISSR AND RAPD ANALYSES

Document Type : Original research

10.12816/ejpb.2019.344255

Abstract

The present investigation was conducted to access the genetic diversity among ten inbred lines of sunflower: Ha89, Ha93, Ha64, Ha101, Ha122, Sha13, Sha14, Sha15, Nsha136 and Nsha140 imported from different origins using RAPD and ISSR techniques. A total of 95 major scorable bands ranging from 117 to 1075 bp were generated from 8 ISSR primers showing 78.8% polymorphism. While, a total of 150 scorable bands ranging from 129 to 2835 bp were produced from 10 RAPD primers detecting 91.3 % polymorphism. Unique bands were produced by both ISSRs and RAPDs. Genetic similarity values were ranged between 51.1- 84.1 % and 38.9- 82.4 % for ISSR and RAPD, respectively. The lowest similarity using ISSR primers was observed between Sha14 and Ha93. While, the lowest similarity using RAPD primers was found between Ha89 and Nsha140. The dendrogram based on ISSR data separated the ten inbred lines into two main clusters at a similarity coefficient of 0.25, while, the dendrogram related to RAPD data separated them into three main clusters. The combined data of RAPD and ISSR showed genetic similarity ranged from 51.0 to 77.8 %. Also, the dendrogram based on the combined data of both ISSR and RAPD displayed considerably similar results to those obtained from individual ISSR analysis.

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